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The pco.flim is the first luminescence decay time microscopy camera using a two tap CMOS image sensor. Synchronized modulation of pixels and stimulated light enables the analysis of decay times in the range of 100 ps to 100 µs. A broad variety of trigger options allows the pco.flim's integration in versatile fields of applications.
It offers all the required generation of frequency domain signals (5 kHz – 40 MHz) and also allows the use of external modulation signals in a limited frequency range (500 kHz – 40 MHz). It has a USB 3.0 interface for image data transfer and control of all camera operation modes. Further, a variety of trigger input / output signals for integration of the camera into any application framework is available. Table 2 gives an overview of the performance data of the camera system.
Key Features
- 100 ps – 100 µs lifetimes measurable
- 5 kHz – 40 MHz modulation frequencies
- 500 kHz – 40 MHz external modulation signals
- modulation signal shape sinusoidal / rectangular
- 1008 x 1008 pixel resolution
- frequency domain FLIM
- USB 3.0 interface
- 39 % quantum efficiency
- 1000:1 dynamic range
- 45 e- rms readout noise
- 90 fps frame rate (2 tap readout)
- 10 ns to 10 s selectable exposure times
- vibration-free water cooling
- special software for measurement and analysis
Video「Fluorescence Lifetime Imaging (FLIM) with a focus on frequency domain FLIM with pco.flim」
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