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Scanning Autocorrelator for Multiphoton Microscopy
Scanning Autocorrelator for Multiphoton Microscopy

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The AA-M provides two simultaneous measurement points: one at the focal plane of the microscope and the other being the point where the optical head of the device is placed, i.e. somewhere before the microscope input. The comparison of the pulse duration value obtained in these two measurements determines the pulse broadening introduced due the dispersion of the microscope’s optical elements. In most cases of application of ultra-short pulses in microscopy it is essential to characterize the temporal and spatial profile of the beam in the focal spot of the microscope. These measurements are vital for any experiment as the shorter is the pulse the higher is the efficiency of the nonlinear imaging process (2-photon excitation) and less excitation energy is needed for successful experiment. Such beam characterization is also necessary when determining exposition of the sample. It ensures image optimization and correct intensity level estimation, as incorrect values may even lead to sample damage. Different models of the autocorrelator cover several wavelength ranges, with the AA-MM model combining the ranges by using 3 interchangeable sets of photodetectors and optics. Two separate ranges can also be covered upon request. Input pulse duration ranges from 20 fs to 12 ps for easy monitoring of different laser systems. The device features USB interface and can be easily hooked up to a PC with Windows OS. The software is supplied with the device and comprises several useful tools. The acquired pulse duration data can be visualized, stored or exported to a .txt or .dat file. Autocorrelation function and final FWHM pulse duration in femtoseconds are calculated and displayed in real-time. Moreover, Gaussian or sech^2 fitting options are enabled, intensity function may also be observed. The statistical viewer feature allows the comparison of data acquired from several separate pulse measurements.

Specifications

Scanning Autocorrelator for Multiphoton Microscopy

Model AA-MV AA-M AA-MR AA-MM
Wavelength range*1 450~700 nm 700~1300 nm 1300~2000 nm 450~2000 nm
Pulse duration range 20 fs~12ps
Input repetition rate >10 KHz
Input polarization*2 Horizontal
Sensitivity >100 mW2(Paverage Ppeak)
Scanning rate 0.1~20 Hz
Optical head size(mm) 210(W)×164(H)×132(L)
Electronic unit size(mm) 225(W)×190(H)×45(L)
External photodetector size(mm) 70(W)×55(H)×16.5(L)
Voltage Voltage:100~220 VAC Single phase:50~60 Hz
  • *1.Model AA-MM may cover either all three ranges (450-2000 nm) or two separate ranges (i.e. 450-1300 or 700-2000) with interchangeable optics and photodetectors (3 or 2 sets included).
  • *2.Vertical upon request
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